Get A Quote

Request A Quote

*We respect your confidentiality and all information are protected.

How to sterilize platelet rich plasma (PRP), that is to be used for stem cell culture?

Contact Us For Test samples

Our team is here to help you find what you need. Let’s get you connected today.

Sterilization of platelet-rich plasma (PRP) is an essential step before it can be used for stem cell culture. Contamination with bacteria, fungi or viruses can cause serious problems during cell culture, affecting the viability and function of stem cells. Here are some steps that can be taken to sterilize PRP for stem cell culture:

Preparation of PRP: PRP can be prepared by standard methods such as centrifugation or automated systems. The PRP should be collected using aseptic techniques in a sterile container to avoid contamination.

Filtration: PRP can be filtered using a sterile filter with a pore size of 0.22 µm or smaller. This will remove any potential contaminants that may be present in the plasma.

Sterilization: PRP can be sterilized by autoclaving or by using a sterilizing agent such as ethylene oxide or gamma radiation. Autoclaving is the preferred method of sterilization for PRP as it is more effective in eliminating microorganisms. Before autoclaving, the PRP should be placed in a heat-resistant, autoclave-safe container. The PRP should be autoclaved at a temperature of 121°C for 15 minutes at a pressure of 15 psi.

Quality control: After sterilization, it is important to perform quality control checks to ensure that the PRP is sterile and suitable for use in stem cell culture. The PRP can be tested for sterility by performing a microbial culture and sensitivity test.

It is important to note that the optimal method of sterilization may depend on the specific protocol used for PRP preparation and the intended use of the PRP. It is always recommended to consult with a medical professional or healthcare provider for guidance on the sterilization and use of PRP for stem cell culture.

Quick Quotation

Related Articles

 About Chronic Pain Chronic pain affects millions worldwide, significantly impacting daily life and overall well-being. Traditional treatments Physiotherapy methods (sound waves, photothermal radiation, vibration, etc.) are usually used, often fall short in providing long-term relief. However, emerging therapies like Platelet-Rich Plasma (PRP) and Peptide Therapy offer promising alternatives.   About PRP Therapy for Chronic Pain PRP vs Peptide Therapy for Chronic Pain
Introduction to Platelet-Rich Therapies Explore the revolutionary world of platelet-rich therapies, understanding how these advancements have redefined wound care and what makes Autologous Matrix of Platelet-Rich Fibrin (PRF) stand out. Understanding Wound Care Delve into the complexities of wound healing, the importance of effective care, and how innovative treatments are changing patient outcomes. What is Autologous Matrix of Platelet-Rich Fibrin in Wound Care 
Introduction to PRF and PRP Exploring the revolutionary advancements in dental regenerative therapies, this article compares Platelet-Rich Fibrin (PRF) and Platelet-Rich Plasma (PRP), shedding light on their uses, benefits, and which stands out as the superior choice in modern dentistry. Understanding PRP: Basics and Benefits Delve into the world of PRP, its extraction process, and PRF vs PRP in Dentistry: Which One is Better

PRP & Needle specialists


Copyright © 2022, KEALOR. Jiangsu, China.