PRP Preparation Procedure

High concentration of autologous Platelet-rich-plasma (PRP)

87%+10%Platelet Recovery Rate

95.5% Platelet Dose Purity

99.4%+0.5% RBC Removes, Takeout WBCs 83%+10%

1.6~4.4 MEAN PRP CONC. FACTOR (X BASELINE)

Simple PRP Extraction

High Concentraction PRP Extraction

Simple PRP Preparation Process

The simple, safe and efficient point of care preparation of

Autologous Platelet Rich Plasma.

PRP preparation (1)KEALOR platelet rich plasma prp tube with separating gel(acd gel, sodium citrate) with biotin ,ha ,activator prp for sale

1. Blood Collection

Connect the blood collection needle to the needle holder, then perform venipuncture, confirm that the line returns blood,

and finally connect the PRP tube.

Please do not connect the PRP tube first

or will lose negative pressure.

Gently shaken back and forth several times to mix the coagulant and blood. ,Ensure blood is mixed with anticoagulantto prevent hemolysis from clottting

KEALOR platelet rich plasma prp tube with separating gel(acd gel, sodium citrate) with biotin ,ha ,activator prp for sale

4. Invert the PRP tube

Gently shake the tube 10~20 times

so that the platelet cells cemented

on the surface of the separation gel are evenly

suspended in the plasma

2. Centrifugation

Insert the KEALOR PRP tubes

into opposite sides of the centrifuge.

If using odd number of tubes，

need use balancing tube .

Close the lid and select the appropriate centrifuge settings for 1,600 RCF and 8 minutes.

5.Extraction of PRP

Use clean syringe to Collect the PRP, The long needle should not be inserted into the separation gel.

3. Take out PRP tube after Centrifuge

Liquid Gold PRP ，PPP upside the gel .

RBC and granulocytes under the gel.

If chylemia, the fluid will be cloudy

Ready to Use

KEALOR PRP is ready for use. pay attention to whether there is turbidity or separation glue separating foreign bodies or other foreign bodies, including the glue plug cutting foreign bodies.

PRP tube 10ML

PLT Concentration* 1.6x

Total Platelets ~2.3 x billion

WBC <5%

RBC <0.5%

PLT Yield >80% (STD Dev. +/-9%)

pH 6.2-7.7

PRP Volume 5~6mL

Average of Several times high concentration extraction . Using 10mL PRP tube, Not removing platelet poor plasma (PPP). Whole Blood platelets counts 209 (106/mL), white blood cell counts of 7.7 (106/mL) and red blood cell counts of 4.7 (109/mL).

PRP tube 15ML

PLT Concentration* 1.8x

Total Platelets ~2.9 x billion

WBC <5%

RBC <0.5%

PLT Yield >80% (STD Dev. +/-9%)

pH6.2-7.7

PRP Volume 6mL

Average of Several times high concentration extraction concentration. Using 15mL PRP tube, removing 2.5mL of platelet poor plasma (PPP) respectively. Whole Blood platelets counts of 209 (106/mL), white blood cell counts of 7.7 (106/mL) and red blood cell counts of 4.7 (109/mL).

PRP tube 22ML

PLT Concentration* 3.3x

Total Platelets ~2.45 x billion

WBC <5%

RBC <0.5%

PLT Yield >80% (STD Dev. +/-9%)

pH 7.2-7.7

PRP Volume 6mL

Average of Several times high concentration extraction . Using 22mL PRP tube, removing 7mL of platelet poor plasma (PPP) respectively. Whole Blood platelets counts of 209 (106/mL), white blood cell counts of 7.7 (106/mL) and red blood cell counts of 4.7 (109/mL).

High Concerntraction PRP Preparation Process

Remove PPP to get high Concentraction PRP

Blood Collection

Centrifugation

Remove PPP

Platelet Resuspension

Ready to Use

1. Blood Collection	Using the butterfly needle and the blood collection set, draw 10cc of venous blood into the KEALOR PRP tube. Invert the filled tube 5 times for mixing.
2.Centrifugation	Insert the KEALOR PRP tubes into opposite sides of the centrifuge. If using an odd number oftubes then the balancing tube needs to be utilized. Spin. Close the lid and select the appropriate centrifuge settings for 1,600 RCF and 8 minutes. Start centrifuge.
3. Remove PPP	Remove the PRP tube from the centrifuge, taking care not to shake it. Collect the supernatant fraction using the syringe & 18G 100mm needle. According to needs, 2.5ml, 1ml and 0.5ml of plasma remain in the lower layer.
4. Platelet Resuspension	Gently invert the KEALOR PRP tube 10 to 20 times.
5. Platelet Preparation	Collect the remaining plasma on the separation gel use new syringe. The KEALOR PRP is ready for use.

NOTE: If step 3 is omitted,this is easy process. You will get 5.5~6.5ml PRP.

Use our 10ml acd gel PRP TUBE get result below

ONCENTRATION FACTOR(X) COMPARED TO BASELINE VALUES IN PERIPHERAL BLOOD

PRP(ML)	Platelets	Red blood cells	White blood cells	Lymphocyte	Granulocyte
5ml ~6ML PRP	1.5X-1.8X	0.00X	0.12X	0.27X	0.01X
2.5 ML PRP	3.2X -4.2X	0.01X	0.24X	0.26X	0.03X
1ML PRP	5X-7X	0.01X	0.45X	1.12X	0.05X
0.5ML PRP	9X-13X	0.01X	0.77X	1.97X	0.05X

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The above are clinical statistics for reference.
Temperature during processing is crucial to prevent platelet activation. recommends 21°C-24°C for centrifugation of blood for obtaining PRP.
1600RCF. “g”‘ （RCF） is the actual force exerted on the contents of the spinning rotor, Revolutions per minute (rpm) is calculated using the following equation
g=r×11.8×10^{-6}×rpm^{2}

Where “g” is the “RCF”, “R” is the radius of the rotor (from centre of rotor to sample) in centimetres， “S” is the speed of the centrifuge in revolutions per minute（rpm）

.